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What is a centrifuge used for?
Source: | Author:Pekybio | Published time: 2022-06-23 | 12 Views | Share:

Centrifuges are used in various laboratories to separate fluids, gases, or liquids based on density. In research and clinical laboratories, centrifuges are often used for cell, organelle, virus, protein, and nucleic acid purification.

An example of centrifuge use in a clinical setting is for the separation of whole blood components. Different assays necessitate serum or plasma, which may be obtained with centrifugation.

Serum is obtained by letting a whole blood sample clot at room temperature. The sample is then centrifuged and the clot is removed, leaving a serum supernatant.

Unlike serum, plasma is obtained from whole blood that is not left to clot, and contains serum along with clotting factors. To obtain plasma, a whole blood sample is collected in tubes treated with anticoagulants. Following centrifugation, cells are removed and plasma supernatant remains.

Centrifuge Rotor Types

There are two very common rotor designs: fixed angle, and swinging bucket. The fixed angle rotor is designed to hold tubes in a fixed position at a fixed angle relative to the vertical axis of rotation (up to about 45°). Centrifugation will cause particles to sediment along the side and bottom of the tube. The swinging bucket design allows the tubes to swing out from a vertical resting position to become parallel to the horizontal during centrifugation. As a result, sediment will form along the bottom of the tube.

Fixed angle rotors are ideal for pelleting applications either to remove particles from a suspension and discard the debris or to recover the pellet, whereas swinging bucket rotors are best for separating large volume samples at low speeds and resolving samples in rate-zonal (density) gradients.

In order to use the centrifuge better, we need to choose the appropriate centrifuge tube.

Centrifuge tubes are used to contain liquids during centrifugation, which separates the sample into its components by rapidly rotating it around a fixed axis. Most centrifuge tubes have conical bottoms, which help collect any solid or heavier parts of the sample being centrifuged.

Pekybio brand centrifuge tubes are available in many sizes for you to choose from. The quality of the centrifuge tube also plays a crucial role in the use of the centrifuge.Of couse pekybio's centrifuge tubes must pass the leak-proof test.
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