My dear, welcome to visit PekyBio !
+86 571 8756 6925
marketing@pekybio.com
What do you know about PCR?
Source: | Author:Pekybio | Published time: 2022-05-05 | 19 Views | Share:
Polymerase chain reaction (PCR) is a molecular biological technology used to amplify specific DNA fragments. It can be regarded as special DNA replication in vitro.

Polymerase chain reaction (PCR) is a molecular biological technology used to amplify specific DNA fragments. It can be regarded as special DNA replication in vitro. The biggest characteristic of PCR is that it can greatly increase the amount of DNA. So if you can isolate a tiny bit of DNA from fossils, the remains of historical figures, hair, skin or blood left behind by a murderer from decades ago, PCR can be used to amplify it and compare it. This is also the power of "trace evidence". The idea was first proposed by Mullis of the United States in 1983. In 1985, he invented polymerase chain reaction, namely simple DNA amplification, which meant the real birth of PCR technology. By 2013, PCR had developed into the third generation of technology. In 1976, Qian Jiayun, a Chinese scientist, discovered the stable Taq DNA polymerase, which also made a fundamental contribution to the development of PCR technology.

 

In PCR experiments, there are many factors that affect the amplification effect, usually including the following: 

1. Primers: Primers are the key to the specific reaction of PCR. The specificity of PCR products depends on the complementary degree of primer and template DNA.

2. The quality and concentration of DNA polymerase and dNTP;

3. The setting of temperature and time, the control of cycle times, etc.

4. Selection of equipment and consumables.

In fact, the choice of consumables does affect the results of experiments to a large extent, which is often overlooked by us. Therefore, before selecting experimental consumables, we need to have a deeper understanding of the characteristics related to PCR consumables. Peky Biotech is committed to providing PCR technology with high quality PCR consumables to meet the target DNA amplification.

 

PEKYBIO PCR:

 

1. Made of 100% pure polypropylene.

2. Extremely thin tube wall design, best sample heat transfer.

3. The cap is easy to open and close. Excellent sealing, effectively reduce evaporation rate. Flat cap and domed cap for your choice.

4. Optimized design of tube, small difference between tubes. Clear tube and White tube for your choice.

5. Free from Pyrogens, DNase, RNase, human DNA, PCR inhibitors.

 

So do you know how to choose more appropriate PCR consumables? 

We will make known, please follow Pekybio.