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Faster PCR Optimization
source: | author:Pekybio | Release time :2019-05-21 | 38 Secondary browse: | Share:
So, you’ve designed PCR primers to amplify your sequence of interest, and you’re ready to go. But unless you have a never-ending supply of template, polymerase, and a thermocycler with a gradient function—not to mention a hefty dose of time and patience—you probably don’t want to spend the next week finding the perfect conditions for your PCR. Here are some tips to get you there faster. (Although, the gradient function is pretty handy.)

So, you’ve designed PCR primers to amplify your sequence of interest, and you’re ready to go.  But unless you have a never-ending supply of template, polymerase, and a thermocycler with a gradient function—not to mention a hefty dose of time and patience—you probably don’t want to spend the next week finding the perfect conditions for your PCR.  Here are some tips to get you there faster.  (Although, the gradient function is pretty handy.)

Melting and Annealing Temperatures
Using Touchdown PCR
Use high quality template!
Extension time
Buffer components
                                           
Written by Kimberly Jasmer-McDonald
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